Chiyo, often called "old-lady Chiyo" or "Granny Chiyo" (チヨバア様, Chiyo-baa-sama) by the Sunagakure villagers, was the village's poison and puppet expert. She and her brother Ebizo were highly revered in Sunagakure and were known as the "Siblings" (御姉弟, Gokyōdai). Chiyo was very knowledgeable when it came to handling poisons and used her skills often during the Second Great Shinobi World War, only to be countered numerous times by Tsunade's medical expertise. This caused Chiyo to hold a grudge against Tsunade, who she came to call "that slug-girl" (あのナメクジ娘, Ano Namekuji-musume).
Chiyo was responsible for sealing the One-Tailed Shukaku inside of Gaara under the orders of the Fourth Kazekage. After this, Chiyo and her brother removed themselves from the affairs of Sunagakure and spent their time together, where Chiyo would play jokes on her brother by pretending she had died just to scare him.
Chiyo also expressed a desire to kill Sakumo Hatake due to the fact that he killed her son and daughter-in-law, thus leaving her grandson Sasori orphaned. After that Chiyo tried to help Sasori ease the pain by caring for him and teaching him the Puppet Technique. Chiyo would later watch Sasori create his first puppets that would resemble his own parents. This was meant to fill the void of his parents dying. When Sasori left Sunagakure he abandoned the puppets, showing that he gave up on them.